The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast
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منابع مشابه
The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast.
Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybrid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on...
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Departamento de Genetica, Universidade Federal do Rio de Janeiro, CP 6801 1, CEP 21944, Rio de Janeiro, Brasil (N.S., R.K., D.E.d.O.); Departamento de Fitopatologia, Universidade Federal Rural do Rio de Janeiro, CP 23851, Rio de Janeiro, Brasil (P.S.T.B.); Departamento de Genetica, Fundagco Oswaldo Cruz, CP 926, Rio de laneiro, Brasil (A.C.P.V.); Departamento de Bioquimica, Universidade Federal...
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Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
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Rpm2p, a protein subunit of yeast mitochondrial RNase P, has another function that is essential in cells lacking the wild-type mitochondrial genome. This function does not require the mitochondrial leader sequence and appears to affect transcription of nuclear genes. Rpm2p expressed as a fusion protein with green fluorescent protein localizes to the nucleus and activates transcription from prom...
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The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2002
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2002000400001